Product #14309

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

CultureWash is designed for washing, exchanging medium, and concentrating cells. It offers flexible parameters and small final volume capabilities when manufacturing cellular therapy products.

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Key features

Features CultureWash
Initial volume 30 to 880 mL
Selectable final volume 8 to 220 mL*
Average CD34+ recovery >80%
Average CD34+ viability 95%

*If product volume reduced to less than 20 mL, CS-900.2 single-use kit and Separation Chamber Holder are needed.

Workflow

Step Details
1 Prepare a washing solution.
2 Prepare a kit with the initial product.
3 Install the kit on the Sepax.
4 The Sepax automatically performs the procedure including:
• Primary dilution
• Osmolarity balancing
• Sedimentation and initial product rinsing
• Supernatant extraction
• Cell resuspension
• Cell extraction into a final product bag.

Product features

  • Dilution ratio: Defines the volume of washing solution used to dilute the initial product for osmolarity balancing.
  • Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
  • Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
  • Main user interface available in: English, French, German, Italian, and Spanish.

Kit / protocol compatibility matrix

Kit / protocol CS-600.1 CS-900.2** CS-490.1
CordWash x - -
SmartWash x - -
CultureWash x x -
Dilution x x x

**The CS-900.2 kit is only validated for initial volumes up to 220 mL. Higher volumes must be validated by the user.

Ordering information

CultureWash: Product #14309

Separation Chamber Holder: Product #6033

Packaging: One box for each protocol, including a USB key with the protocol installed and a user installation guide.