Product #14300

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

NeatCell is a protocol designed for isolation by means of a density gradient of the mononuclear cell (MNC) fraction from various cellular products into a small final volume of 8-20 mL. Alternatively, a fixed final volume of 45 mL can be chosen. In both cases, optimal granulocyte and Red Blood Cells (RBC) depletion is achieved.

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Key features

NeatCell features  
Initial volume 30 to 120 mL
Selectable final volume 8 to 20 mL, or 45 mL
Processing time ~1h15 minutes
MNC recovery > 52%
RBC depletion > 99%

Workflow

Step Details
1 Prepare a kit with density gradient medium (via microbial filter), washing solution, and a syringe for collecting the final product (if applicable).
2 Connect initial product to the kit.
3 Install the kit on the Sepax.
4 The Sepax automatically performs the procedure including:
• Priming
• Isolation via a density gradient medium
• Washing
5 Collect purified mononuclear cells from the final product bag or via a syringe.

Product features

  • Automated procedure comprising of all required steps.
  • Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
  • Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
  • Main user interface available in: Chinese, English, French, German, Italian, and Spanish.

Accessory for small final volume option

Use the separation chamber holder for the last steps of the procedure when collecting the final cells in a pre-connected syringe.

Kit / protocol compatibility matrix

Kit / protocol CS-490.1 CS-470.1 CS-900.2 CS-430.1*
PeriCell x - - x
SmartRedux x - - -
ReadyCell - x - -
NeatCell - - x -
Adipose - - x -

*Maximum initial volume limited to 600 mL.

Ordering information

NeatCell: Product #14300

Packaging: One box for each protocol, including a USB key with the protocol installed and a user installation guide.